The Cameraman’s Revenge is a stop motion animation featuring real (dead) insects from (1912):

The Cameraman's Revenge (1912) is a 13-minute experimental Russian stop-motion animated short directed and written by Wladyslaw Starewicz. This animation was one-of-a-kind when it was created for its use of real dried-out insects as characters. The film includes beetles, grasshoppers, dragonflies, and other fine specimens. — Read the rest